FEMTO3D Atlas Multiphoton microscope

Reference number: FEM-FEMTO3DAtlas

The FEMTO3D Atlas enables its users to scan cells and neuronal, dendritic, or other biological processes in 3D, up to a million times faster than classical scanning methods, at a preserved two-photon resolution.

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Description

Atlas combines an blend of high-tech science, engineering, refinement in 3D measurements.

The Innovation Award winner FEMTO3D Atlas offers an All-in-One two-photon system for research scientists working in neuroscience and many other life science fields: it incorporates traditional galvo and resonant scanner-based microscope imaging techniques and combines them with a 3D imaging feature thanks to the unique acousto-optic scanner.

FEATURES AND BENEFITS

3D imaging extension for any existing microscopes and individual system
Simultaneous read out of neural activity on both the somatic and dendritic scales
Increased measurement speed and signal-to-noise ratio by several orders of magnitude
3D Network imaging of over 2000 soma distributed in 3D
Imaging of dendritic branches through layers
Simultaneous imaging of over thousands of spines
Unique, flexible scanning methods supporting in vivo and in vitro measurements
In vivo 3D motion correction by specialized scanning patterns
High-speed 3D random-access ROI scanning: scanning speed up to 30 kHz to any points in 3D
High-speed raster scanning: 40 fps at 510×510, and even 3000 fps at reduced FOV
Improved excitation efficiency for large 3D scanning volume with GECIs
Automatic wavelength tuning between 750 – 1050 nm
Functional imaging correlated with electrophysiological data
Calcium imaging during behavior
3D random-access photo stimulation for optogenetics