Multicolour LED sources for microscopic fluorescence excitation

Single Multiband Wavelength Excitation for a Mercury Free Microscope
  • Single Multiband Wavelength Excitation for a Mercury Free Microscope
  • Single Multiband Wavelength Excitation for a Mercury Free Microscope

Reference number: BBO-NIJI

Single multiband wave excitation is a revolutionary length innovation in mercury-free microscopy that takes advantage of recent advances in lighting technology. With a much lower environmental impact, this cost-effective modular LED source with single or multi-band wavelength illumination is groundbreaking.


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Single Multiband Wavelength Excitation for a Mercury Free Microscope

The niji is a revolutionary innovation in mercury-free microscopy, taking advantage of recent advances in lighting technology. With a much smaller environmental impact, this low cost modular LED source with single or multiband wavelength illumination is leading the way. We care about the environment and are trying to help users who care too. The niji can help you to remove dangerous toxins and has far less power consumption than damaging mercury lamps, helping to do your bit toward the environmental impact of research. Weve tried to use the latest technology to reduce the impact of production but also used old technology and made our packaging recyclable or degradable where possible. You may also be able to benefit from a sustainability grant from your institution to help you switch to new green tech.

But, why should I choose a niji?

  • Individually addressable, individually filtered LED channels

  • Fully compatible with microscopes and microscope software

  • Individual excitation filters

  • Constant current and optical power LED drive modes

  • User-changeable LEDs and filters

  • Control individually or simultaneously


Wavelenght (nm) Bandwidth
FWHM (nm)
Typical Optical
Power (mW)
Recommended Filter Sets Product
7-colour LED source
(complete, populated unit)
UV 395 10 120 LED-DAPI-A-000 LED Module BBO-LED-390-001
445 18 200 LED-CFP-A-000 BBO-LED-440-001
Blue 470 19 185 LED-FITC-A-000 BBO-LED-470-001
Cyan 505 31 90 LED-YFP-A-000 BBO-LED-510-001
556 62 550



Red 635 18 225 LED-Cy5-A-000 BBO-LED-630-001
725 38 70 LED-Cy7-A/B-000 BBO-LED-720-001

Microscope Adapter Product Codes
Nikon adapter  BN7-ADT-N1-001
Leica adapter  BN7-ADT-L1-001
Olympus adapter  BN7-ADT-O1-002c
Zeiss adapter  BN7-ADT-Z1-001
Other Accessories
BNC breakout cable  BN7-BNC-7-001
Modular Channels Up to seven channels, user-fit*
Sources Solid state LED
Wavelength Range 380 730 nm available
Noise Level Approx. 47 dB
Bandpass Filters Individual bandpass filter holders for each channel
Light Interface Liquid light guide with microscope collimators, available separately
Connection USB-A (powered), USB-B, RS232 (serial), and TTL via 15-way connector
Operation Control via dedicated software, MicroManager, MicroManager 2, tablet, or third-party software
Programmable Setup XML upload for complete flexibility
Optical Safety Key switch, light guide, and lid-off interlock
Power Consumption Standby ~5W, Single colours: green ~40W, all other colours ~18W, All 7 colours on ~80W
Dimensions & Weight 315 x 288 x 107 mm, 4.7 kg
Warranty 12  months, optional 3 years
Operating requirements:
Line Voltage 110V & 240V AC
Line Frequency 50/60 Hz
Power Consumption 350 VA
Operating Temperature 10 to 40 °C
Storage Temperature -10 to 60 °C
Humidity 40% to 70% RH

Cost Saving Chart

Cost Mercury lamp Metal Halide Lamp LED light engine Niji (7 channel)
Bulbs used in niji lifetime (20,000 hours) 80 pieces with a
lifetime ~250 h

8 pieces with a
lifetime ~ 2500 h

Cost of bulbs used

~ 290 per bulb

total: 23 294,60

~580 per bulb 
total: 4658,92
Energy consumed in 20,000 hours usage 0.140 kWh/h 2,800 kWh 0.22 kWh/h 4,400 kWh

0.04 kWh/h

800 kWh

Electricity cost in 20,000 hours usage (12.4p/kWh) 404,39 635,48 115,54
Overall disposal cost ~4,08 per bulb 326 ~4,08 per bulb 32 N/A
Cost over 20,000 hours 24 025,12 5327,01 115,54
Cost per hour 1,20 0.26 0.006
(Exchange rate from June 16, 2021: 1 GBP = 1.16120 EUR)